Catalog Number - 68140-10P

Cogent NP Amino™ is a versatile normal phase and HILIC stationary phase designed for the separation of highly polar compounds that are often difficult to retain using traditional reversed-phase methods. Manufactured on ultra-high purity silica with a propyl amino bonded phase and supplied without endcapping, this chemistry provides excellent selectivity for carbohydrates, sugars, organic acids, and other polar analytes. The stationary phase is commonly used as an alternative for USP L8 methods and is suitable for both Normal Phase and HILIC applications.

68140-10P is a UHPLC column packed with 1.8 µm NP Amino™ particles featuring a 100 Å pore size in a 4.6 mm ID x 100 mm format. The 100 mm column length provides increased resolution and peak capacity compared to shorter 50 mm columns, while the sub-2 µm particle technology delivers exceptional efficiency and chromatographic performance. This column is specifically designed for high-pressure UHPLC operation and provides optimum performance when used on UHPLC systems capable of handling the elevated pressures generated by 1.8 µm particles. Typical operating flow rates are approximately 1.0–2.0 mL/min depending on mobile phase composition and system pressure limitations.

This column is particularly effective for sugars, carbohydrates, negatively charged compounds, organic acids, and compounds containing carboxylic acid functionality. Suitable for both Normal Phase and HILIC applications, it is an excellent choice when enhanced retention of polar compounds and increased chromatographic resolution are required.

Key Benefits

• NP Amino™ stationary phase

• USP L8 equivalent chemistry

• Propyl amino bonded phase

• Ultra-high purity silica support

• Suitable for Normal Phase chromatography

• Suitable for HILIC applications

• Designed for UHPLC applications

• High-efficiency 1.8 µm particles

• Exceptional peak capacity

• Increased chromatographic resolution

Typical Applications

• UHPLC analysis

• USP L8 methods

• HILIC separations

• Normal Phase chromatography

• Carbohydrate analysis

• Sugar analysis

• Organic acid analysis

• Polar compound analysis

• Pharmaceutical analysis

• Method development

Specifications

• Product Type: UHPLC Column

• Stationary Phase: NP Amino™

• USP Classification: L8

• Chromatography Mode: Normal Phase / HILIC

• Bonded Phase: Propyl Amino

• Endcapping: Not Endcapped

• Particle Size: 1.8 µm

• Pore Size: 100 Å

• Column Dimensions: 4.6 mm ID x 100 mm

• Length: 100 mm

• Typical Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Important Notes

• Specifically designed for UHPLC operation and high-pressure chromatography

• Delivers maximum performance when used on a UHPLC system designed for sub-2 µm particle columns

• While compatible HPLC systems may be used in some applications, the column is engineered primarily for UHPLC operation

• Sub-2 µm particles generate substantially higher backpressure than conventional HPLC columns

• Packed and shipped in Normal Phase solvents

• Suitable for both Normal Phase and HILIC chromatographic modes

• Excellent for sugars, carbohydrates, organic acids, and other highly polar compounds

• The 100 mm length provides greater resolution and peak capacity than 50 mm columns

• Samples should be filtered through a 0.2 µm syringe filter prior to injection

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